Production of mature mRNA in eukaryotes is a multi-step process that requires cooperation between numerous factors. The elongation step of transcription is generally described as the process through which the polymerase molecule traverses the coding region of the gene after clearing the promoter region. To facilitate this event, transcription elongation factors associate with RNA polymerase II (Pol II) on the body of the gene and traverse through this region with kinetics similar to that of Pol II. These elongation factors help the RNA polymerase to negotiate the nucleosomes and in some cases provide linkage to concurring or subsequent processing events.
To identify factors that are important for transcription from heat shock genes, our lab started a functional RNAi screen on a subset of candidate genes. One of the factors that emerged from the initial rounds of the screen was the elongation factor Spt6. Using a battery of approaches, I am currently studying the role that this factor plays in different aspects of transcription elongation from heat shock genes.
- M.Sc. in Biology (2003), Ball State University, Muncie, IN.
- B.Sc. in Biology (1999), Shiraz University, Shiraz, Iran.
- Yao J, Ardehali MB, Fecko CJ, Webb WW, Lis JT. (2007) "Intranuclear distribution and local dynamics of RNA polymerase II during transcription activation." Mol Cell. 28(6):978-90. (PubMed)
- Adelman K, Wei W, Ardehali MB, Werner J, Zhu B, Reinberg D, Lis JT. (2006) "Drosophila Paf1 modulates chromatin structure at actively transcribed genes." Mol Cell Biol. 26(1):250-60. (PubMed)
- (2005) Poster abstract for the 2005 Cold Spring Harbor meeting, Mechanisms of Eukaryotic Transcription.