In vivo imaging of subcellular structures in plant cells
The images and movies shown below supplement the following review articles:
Köhler, R.H. 1998. GFP for in vivo imaging of subcellular structures in plant cells. Trends in Plant Science3:317-320
Hanson, M.R. and R.H. Köhler. 2001. GFP imaging: methodology and application to investigate cellular compartmentation in plants. J Exp Bot., 52(356):529-39.
QuickTime movies can be downloaded by clicking on a link. Free movie players for different types of computers can be found at http://quicktime.apple.com. Images are taken with a BioRad MRC-600 confocal laser scanning microscope with a K1/K2 filter set. For color images, red chlorophyll autofluorescence and green GFP fluorescence were collected in separate channels, imported into Confocal Assistant, NIH-Image or Adobe Photoshop and merged. The green and red color are pseudo-colors that resemble the fluorescence that is observable by epifluorescence microscopy.
1. Chloroplast stromule movie
Tobacco mesophyll cell expressing GFP targeted to the chloroplast stroma. Tubules extending from the chloroplast surface are visible. Scale bar = 10 µm. Dynamic movements of chloroplast tubules are visible in a Quicktime movie made from a time-lapse series. Images were taken at 5 sec. intervals. The movie runs at 20 times normal speed.
- Color movie with red chlorophyll and green GFP fluorescence (1.6 MB).
- Black and white movie with GFP only (3MB).
2. Mitochondria movies
Tobacco suspension cell expressing GFP targeted to mitochondria. Projection of 58 images taken at 1 µm intervals along the optical z-axis through a complete cell. Scale bar = 10 µm.
- Browse through an image stack of a complete cell (3.5 MB) or
- view of a rotating D-reconstruction of a cell (0.84 MB).
3. Guard cells of a transgenic tobacco plant expressing GFP targeted to mitochondria.
Projection of 40 images taken at 0.2 µm intervals along the optical z-axis. Scale bar = 10 µm. Chloroplasts show red chlorophyll autofluorescence and mitochondria green GFP fluorescence.
To see other examples of GFP localized within plants and plant cells, see the web site produced by the lab of Jim Haseloff at the University of Cambridge (http://www.plantsci.cam.ac.uk/Haseloff/).