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Kwok and Hanson (2004) supplementary information

In vivo imaging of plastids and stromules in plant cells

The images and movies shown below supplement the following review article: Kwok, EY and Hanson, MR. 2004. Stromules and the dynamic nature of plastid morphology. Journal of Microscopy. 214: 124-137.
Quick Time movies can be downloaded by clicking on a link. Free movie players can be found at http://quicktime.apple.com.

All images were collected from transgenic Nicotiana tabacum expressing plastid-targeted GFP. Images were collected via laser scanning confocal microscopy with excitation at 488nm and emission collected between 500nm and 600nm. For each image, GFP fluorescence is pseudocolored green and chlorophyll autofluorescence is pseudocolored red. Transmitted laser light was used to create DIC (Nomarski) background images which are pseudocolored blue.

Movie 1. Low magnification of dark-grown hypocotyl epidermal cells.

Irregular shaped plastids with stromules are distributed throughout the cell cortex. 20 images were collected at 5 second intervals. Movie plays ~30X real-time. 1 pixel = 189nm.

Movie 2. High magnification of dark-grown hypocotyl epidermal cells.

Plastids and stromules are clustered around a nucleus. 60 images were collected at 5 second intervals. Movie plays ~30X real-time. 1 pixel = 116nm.

Movie 3. Low magnification of light-grown hypocotyl epidermal cells.

Chloroplasts with short thick stromules are scattered throughout the cell cortex. Cell walls of epidermal cells are visible in blue DIC background image. 20 images were collected at 5 second intervals. Movie plays ~30X real-time. 1 pixel = 189nm.

Movie 4. High magnification of light-grown hypocotyl epidermal cells.

Chloroplasts with stromules are clustered around a nucleus. 30 images were collected at 30 second intervals. Stromules appear to retract into chloroplast bodies over time, likely in response to the extended imaging procedure. Movie plays ~180X real-time. 1 pixel = 134nm.